Agar Protocols
My experiences with cultivating mushroom colonies on agar plates, sterilizing & petri dishes.
Because I needed some reliable source of DNA for sampling, I had to learn how to control the environment variables for cultures of mushrooms on petri dishes in my lab.
This was a first time success and contamination rate was very low. (1 out of 20 gone bad)
I have learned to spot contaminations early, transplant the most rhyzomorphic parts while keeping the sterile protocols.
I have also tried different mushrom species and clone directly from the tissue and from dried caps I had collected the spores for later storage.
MAE Recipe #1
The best recipe that has worked for me so far.
- 10g Agar Agar
- 7.5g Malt (note: if liquid candid, add more)
- 500 ml boiling purified water
notes:
- stir between sterilization to disallow sugar to solidify
- sterilization: 20min @ 15psi
If you have electric cooker like Tesla Cooker, pressure might fluctuate so I go for aprox. 30 min total)
Petri Dish Sterilization: 30 min @ 15psi
Agar Agar Sterilization: 30 min @ 15psi